The message "No such directory or folder" is coming from 2 rules i.e. Rule-File-Connect.Invoke and Rule-File-Connect.pyTestConnectivity activities step 2. Along with this message we are printing the path which is available in the parameter. So you can probably trace the agent activity and try to analyze what is different with the normal execution of the activity. Also check the logs by adding some debug statements in this code.
Hope this might help you to troubleshoot this issue.